Laboratory attitudes and practices in the conservation of biological tissues for research purposes in Cote d'Ivoire
Abstract
Background : Since 2009, Côte d'Ivoire has had a biobank, which now houses the ECOWAS regional biobank. This biobank intends to set up a biological tissue bank for research. In Côte d'Ivoire, several public and private structures handle biological tissues. However, we do not know which ones are involved in the conservation of biological tissues and their related procedures. To ensure optimal conservation of biological tissues at the biobank of Côte d'Ivoire, it is necessary to conduct a study on the state of the art of tissue preservation in Côte d'Ivoire. The objective of our work is to know the attitudes and practices of biological tissue preservation in Côte d'Ivoire.
Methods : This is a descriptive study carried out in biological analysis laboratories in the south of the Ivory Coast.
Results : A total of 66 laboratories agreed to answer the questionnaire that was proposed to them. Among them, only 21.21% kept biological tissues, of which 42.86% were human tissues, 35.71% animal tissues and 21.43% plant tissues. Human tissuesz were stored in formalin at room temperature for a period ranging from 2 weeks to 6 months. Animal biopsies and surgical specimens were stored in formalin and at -80°C for an indefinite period of time. Plant tissues were stored at room temperature and +4°C for an indefinite period of time.
Conclusion : The conservation quality of biological tissues still needs to be improved.
Keywords
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Herpel, E., Koleganova, N. and Schirmacher, P. (2008) [Tissue bank of the National Centre for Tumour Disease. An innovative platform for translational tumour]. Pathologe, 29, 204-209. doi:10.1007/s00292-008-1067-2
Riegman, P.H.J., Dinjens, W.N.M. and Oosterhuis, J.W. (2007) Biobanking for Interdisciplinary Clinical Research. Pathobiology, 74(4), 239-244. doi:10.1159/000104451
Riegman, P.H.J., Morente, M.M., Betsou, F., de Blasio, P. and Geary, P. (2008) Biobanking for better healthcare. Molecular Oncology, 2(3), 213-222. doi:10.1016/j.molonc.2008.07.004
Stege, A. and Hummel M. (2008) [Experience with establishment and operation of a biobank]. Pathologe, 2, 214-217. doi:10.1007/s00292-008-1043-x
Barr, M., Souan, L., MacGabhann, P. et al. (2014) The Establishment of an ISO Compliant Cancer Biobank for Jordan and its Neighboring Countries Through Knowledge Transfer and Training. Biopreservation and Biobanking, 12(1), 3-12. doi:10.1089/bio.2013.0072
Le Guellec, S., Lacroix-Triki, M., Delord, J-P., Ben Allal, C. and Rochaix P. (2009) Les nouveaux fixateurs tissulaires. Revue Francophone des Laboratoires, 2009(408), 25-32. doi:10.1016/S1773-035X(09)70047-4
Hofman, P. (2009) Quels fixateurs ? Pour quelles indications ? Principaux critères d’évaluation des méthodes alternatives à la fixation par le formol. Revue Francophone des Laboratoires, 2009(408), 45-48. doi:10.1016/S1773-035X(09)70050-4
Srinivasan, M., Sedmak, D. and Jewell, S. (2002) Effect of Fixatives and Tissue Processing on the Content and Integrity of Nucleic Acids. The American Journal of Pathology, 161(6), 1961-1971. doi:10.1016/S0002-9440(10)64472-0
Masuda, N., Ohnishi, T., Kawamoto, S., Monden, M. and Okubo K. (1999) Analysis of chemical modification of RNA from formalin-fixed samples and optimization of molecular biology applications for such samples. Nucleic Acids Research, 27(22), 4436-4443. doi:10.1093/nar/27.22.4436
Lou, J.J., Mirsadraei, L., Sanchez, D.E. et al. (2014) A review of room temperature storage of biospecimen tissue and nucleic acids for anatomic pathology laboratories and biorepositories. Clinical Biochemistry, 47(4-5), 267-273. doi:10.1016/j.clinbiochem.2013.12.011
Yasojima, K., McGeer, E.G. and McGeer, P.L. (2001) High stability of mRNAs postmortem and protocols for their assessment by RT-PCR. Brain Res Brain Res Protoc, 8(3), 212-218. doi:10.1016/s1385-299x(01)00119-2
Leonard, S., Logel, J., Luthman, D., Casanova, M., Kirch, D. and Freedman, R. (1993) Biological stability of mRNA isolated from human postmortem brain collections. Biol Psychiatry, 33(6), 456-466. doi:10.1016/0006-3223(93)90174-c
Chu, T-Y., Hwang, K-S., Yu, M-H., Lee, H-S., Lai, H-C. and Liu, J-Y. (2002) A research-based tumor tissue bank of gynecologic oncology: characteristics of nucleic acids extracted from normal and tumor tissues from different sites. Int J Gynecol Cancer, 12(2), 171-176. doi:10.1046/j.1525-1438.2002.01085.x
Ma, Y., Dai, H. and Kong, X. (2012) Impact of warm ischemia on gene expression analysis in surgically removed biosamples. Analytical Biochemistry, 423(2), 229-235. doi:10.1016/j.ab.2012.02.003
Sherwood, K.R., Head, M.W., Walker, R., Smith, C., Ironside, J.W. and Fazakerley, J.K. (2011) RNA integrity in post mortem human variant Creutzfeldt-Jakob disease (vCJD) and control brain tissue: RNA integrity in human vCJD brain tissue. Neuropathology and Applied Neurobiology, 37(6), 633-642. doi:10.1111/j.1365-2990.2011.01162.x
Atz, M., Walsh, D., Cartagena, P. et al. (2007) Methodological considerations for gene expression profiling of human brain. Journal of Neuroscience Methods, 163(2), 295-309. doi:10.1016/j.jneumeth.2007.03.022
Tissus végétaux préservés en vue d’études génétiques (2019). Muséum national d’Histoire naturelle. https://www.mnhn.fr/fr/collections/ensembles-collections/botanique/tissus-vegetaux-preserves-vue-etudes-genetiques. Accessed December 19, 2019.
DOI: https://doi.org/10.23954/osj.v5i4.2597
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