Androgen derivatives affect immunopresence but not activity of antioxidant enzymes in pancreatic islets during diabetogen-induced apoptosis

Martin Palomar-Morales


The onset of diabetes mellitus involves oxidative stress. Previous reports from our laboratory have shown that androgens, but not estrogens or progestogens, could prevent the apparition of apoptosis in beta-cell induced by streptozotocin in early stages of diabetes induction in rat; these effect was related with changes in antioxidant enzymes presence. The aim of this study was to evaluate the participation of scavenging enzymes in beta-cells protection under androgen derivatives, and their cytoprotective mechanism. Adult male rats were orchidectomyzed, and treated with androgens after STZ administration. Pancreatic catalase and superoxide dismutase-1 and -2 were analyzed by immunohistochemistry and TUNEL assay. In another experimental focusing, pancreatic islets were obtained from intact male rats, and incubated with alloxan in order to induce ROS production, in the presence of androgen derivatives, and the activities of catalase, superoxide dismutase and glutathione peroxidase were measured in cell lysates. Androgen treatment affects immunopresence of the antioxidant enzymes in pancreas, but not their activity in islets, suggesting that scavenging enzymes could be affected during beta-cells apoptosis.


Pancreatic islets, scavenging enzymes, androgen derivatives, apoptosis, immunohistochemistry

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